Joalo ka ha baetsi ba meralo ea litoropo ba hlophisa phallo ea likoloi ka hloko metseng ea litoropo, lisele li laola ka kelo-hloko motsamao oa limolek'hule ho pholletsa le meeli ea tsona ea nyutlelie. E sebetsa joalo ka balebeli ba liheke tse nyane haholo, li-nuclear pore complexes (NPCs) tse kentsoeng ka har'a lera la nyutlelie li boloka taolo e nepahetseng holim'a khoebo ena ea limolek'hule. Mosebetsi o matla o tsoang ho Texas A&M Health o senola khetho e tsoetseng pele ea sistimi ena, e ka fanang ka maikutlo a macha mabapi le mafu a neurodegenerative le nts'etsopele ea mofets'e.
Phetoho Tracking ea Limolek'hule Pathways
Sehlopha sa liphuputso sa Dr. Siegfried Musser sa Texas A&M College of Medicine se qalile liphuputso mabapi le ho tsamaea ka potlako, ntle le ho thulana ha limolek'hule ka har'a mokoallo oa nucleus oa li-membrane tse peli. Phatlalatso ea bona ea bohlokoa ea Nature e fana ka lintlha tsa phetoho e entsoeng ke theknoloji ea MINFLUX - mokhoa o tsoetseng pele oa ho nka litšoantšo o khonang ho nka metsamao ea limolek'hule tsa 3D tse etsahalang ka li-milliseconds ka sekala ka makhetlo a ka bang 100,000 ho feta bophara ba moriri oa motho. Kgahlanong le menahano ya pele mabapi le ditsela tse arohaneng, dipatlisiso tsa bona di bontsha hore ditsamaiso tsa ho reka le ho romela thepa ya nyutlelie di arolelana ditsela tse ngatanyana ka hara sebopeho sa NPC.
Lintho Tse Sibolotsoeng Tse Hlollang Phephetsa Mehlala e Teng
Maikutlo a sehlopha a senotse mekhoa e neng e sa lebelloa ea sephethephethe: limolek'hule li tsamaea ka mahlakoreng a mabeli likanaleng tse patisaneng, li ntse li tsamaea li pota-pota ho e-na le ho latela litselana tse inehetseng. Hoa makatsa hore ebe likaroloana tsena li tsepamisitse maikutlo haufi le marako a kanale, li siea sebaka se bohareng se se na motho, ha tsoelo-pele ea tsona e lieha haholo - hoo e ka bang makhetlo a 1,000 butle ho feta motsamao o sa sitisoeng - ka lebaka la marang-rang a protheine a thibelang ho theha tikoloho e nang le syrupy.
Musser o hlalosa sena e le "boemo bo thata ka ho fetisisa ba sephethephethe bo ka nahanoang - ho phalla ha litsela tse peli liphasejeng tse moqotetsane." Oa lumela, “Liphuputso tsa rōna li fana ka menyetla e sa lebelloang e sa lebelloang, e senolang ho rarahana ho hoholo ho feta likhopolo tsa rōna tsa pele.”
Katleho Ho sa Tsotellehe Litšitiso
Ho khahlisang, litsamaiso tsa lipalangoang tsa NPC li bonts'a ts'ebetso e makatsang leha ho na le mathata ana. Musser o hakanya, "Bongata ba tlhaho ba li-NPC bo ka thibela ts'ebetso ea ho sebetsa ho feta tekano, ka ho fokotsa tšitiso ea tlholisano le likotsi tsa ho thibela." Sebopeho sena sa tlhaho se bonahala se thibela gridlock ea limolek'hule, Mona'ho na le mofuta o ngotsoeng bocha o nang le li-syntax tse fapaneng, sebopeho le likheo tsa lirapa ha o ntse o boloka moelelo oa mantlha:
Sephethephethe sa Limolek'hule se Tsamaisa Tsela: Li-NPC li Senola Litsela Tse Patiloeng
Sebakeng sa ho tsamaea ka kotloloho ho NPC's central axis, limolek'hule li bonahala li tsamaea ka har'a e 'ngoe ea litsela tse robeli tse khethehileng tsa lipalangoang, e 'ngoe le e' ngoe e lekanyelitsoe ho sebopeho se builoeng haufi le pore.'s lesale la ka ntle. Tokisetso ena ea sebaka e fana ka maikutlo a mokhoa oa motheo oa meralo o thusang ho laola phallo ea limolek'hule.
Musser oa hlalosa,“Le hoja li-pores tsa nyutlelie tsa tomoso li tsejoa hore li na le'plug e bohareng,'hantle-ntle sebopeho sa eona e ntse e le sephiri. Liseleng tsa motho, tšobotsi ena hasn'E 'nile ea hlokomeloa, empa likarolo tsa ts'ebetso lia utloahala-le pore's center e ka sebetsa joalo ka tsela e ka sehloohong ea ho romela thepa ea mRNA.”
Khokahano ea Mafu le Mathata a Phekolo
Ho se sebetse hantle ho NPC-tsela ea bohlokoa ea cellular-e hokahane le mathata a tebileng a methapo ea kutlo, ho kenyelletsa le ALS (Lou Gehrig's lefu), Alzheimer's, le Huntington's lefu. Ho feta moo, ts'ebetso e ntseng e eketseha ea ho rekisa NPC e hokahane le tsoelo-pele ea mofets'e. Leha ho shebana le libaka tse itseng tsa pore ho ka thusa ho thibela litšitiso kapa ho liehisa lipalangoang tse feteletseng, Musser o hlokomelisa hore ho senya ts'ebetso ea NPC ho na le likotsi, ha ho nahanoa ka karolo ea eona ea bohlokoa ho pholoheng ha lisele.
“Re tlameha ho etsa phapang lipakeng tsa mefokolo e amanang le lipalangoang le litaba tse amanang le NPC's kopano kapa disassembly,”oa hlokomela.“Le hoja likamano tse ngata tsa mafu li ka 'na tsa oela sehlopheng sa ho qetela, mekhelo e teng-joalo ka liphatsa tsa lefutso tsa c9orf72 ho ALS, tse etsang likarolo tse thibelang pore.”
Litaelo tsa ka moso: Ho etsa 'mapa oa Litsela tsa Liphahlo le Litšoantšo tsa Live-Cell
Musser le mothusi Dr. Abhishek Sau, ho tsoa Texas A&M's Joint Microscopy Lab, rera ho batlisisa hore na mefuta e fapaneng ea thepa-joalo ka li-subunits tsa ribosomal le mRNA-latela litsela tse ikhethileng kapa u kopane litseleng tse arolelanoang. Mosebetsi oa bona o tsoelang pele le balekane ba Jeremane (EMBL le Abberior Instruments) le bona ba ka fetola MINFLUX bakeng sa ho nka litšoantšo ka nako ea sebele liseleng tse phelang, ho fana ka maikutlo a e-s'o ka a e-ba teng ka matla a lipalangoang tsa nyutlelie.
E tšehetsoa ke lichelete tsa NIH, thuto ena e tsosolosa kutloisiso ea rona ea lisebelisoa tsa cellular, e bontšang kamoo li-NPC li bolokang taolo motseng o phetheselang oa microscopic oa khubu.
Nako ea poso: Mar-25-2025
